miércoles, 15 de abril de 2015

Third Trimester

Due to the long time until deadline of the project, we are going to collect more data in order to improve our experiment. We would repeat the whole - now that we know how to do it, the procedure would be much faster. The Monday 20th we would prepare the solution just after the trip to Río Tinto. The week of the 29th and of the 6th we would finish collecting the final data. From then to the beginning of July we would conclude and evaluate our project.

martes, 3 de marzo de 2015

Results

Ø  RESULTS

Absorbance (or optical density) is the ratio between the light intensity which strikes the culture (I­o) and the intensity transmitted to the culture (I). This value is derived from log (Io/I). When the cellular concentration increases, the culture would become more turbid, meaning that less light would pass through the liquid a more of it would be absorbed.
Absorbance doesn't have any units because it’s the ratio of the amount of light that passes through a solution compared to the amount of light that is passed into it.
We measured the absorbance of the cultures at a 600nm wavelength because it is a standardized measurement to verify absorbance in solutions.
1)     Culture #1: 30mL of water
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
0,01849
593,92
0,01840
596,88
0,01860
599,84
0,01840
602,8
0,01811
605,76
0,01806
608,72
0,01746
611,68
0,01769
614,64
0,01775
Wavelength (±0,01nm)
Absorbance
590,96
0,93931
593,92
0,93769
596,88
0,92714
599,84
0,92588
602,8
0,92284
605,76
0,92763
608,72
0,92125
611,68
0,92367
614,64
0,91083
Absorbance: 0,92284 – 0,01811 = 0,90473









2)     Culture #2 (0,0067 g/ml)
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
0,00593
593,92
0,00625
596,88
0,00512
599,84
0,00489
602,8
0,00384
605,76
0,00255
608,72
0,00222
611,68
0,00200
614,64
0,00327
Wavelength (±0,01nm)
Absorbance
590,96
0,92234
593,92
0,91922
596,88
0,91013
599,84
0,90863
602,80
0,90400
605,76
0,91133
608,72
0,90636
611,68
0,90692
614,64
0,89399
Absorbance: 0,90400-0,00384 =0,90016

3)     Culture #3  (0,0067)
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
0,02991
593,92
0,02992
596,88
0,02944
599,84
0,02905
602,8
0,02873
605,76
0,02822
608,72
0,02720
611,68
0,02662
614,64
0,02596
Wavelength (±0,01nm)
Absorbance
590,96
1,02914
593,92
1,02800
596,88
1,01537
599,84
1,01366
602,8
1,00966
605,76
1,01472
608,72
1,01065
611,68
1,00958
614,64
0,99727
Absorbance: 1,00966-0,02873 = 0,98093

4)     Culture #4 (0,0100 g/ml)
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
0,01535
593,92
0,01630
596,88
0,01590
599,84
0,01566
602,8
0,01476
605,76
0,01356
608,72
0,01211
611,68
0,01147
614,64
0,01231
Wavelength (±0,01nm)
Absorbance
590,96
1,01810
593,92
1,01512
596,88
1,00610
599,84
1,00249
602,8
0,99841
605,76
1,00689
608,72
1,00089
611,68
1,00156
614,64
0,98540
Absorbance: 0,99841-0,01476 = 0,98365


5)     Culture #5 (0,0144 g/ml)
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
0,01227
593,92
0,01213
596,88
0,01111
599,84
0,01134
602,8
0,01020
605,76
0,00933
608,72
0,00837
611,68
0,00787
614,64
0,00837
Wavelength (±0,01nm)
Absorbance
590,96
1,02359
593,92
1,02241
596,88
1,01160
599,84
1,01166
602,8
1,00764
605,76
1,01265
608,72
1,00480
611,68
1,00746
614,64
0,99321
Absorbance: 1,00764-0,01020= 0,99744

6)     Culture #6 (0,0256 g/ml)
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
1,12351
593,92
1,12525
596,88
1,10956
599,84
1,10923
602,8
1,10365
605,76
1,11113
608,72
1,10340
611,68
1,10152
614,64
1,08512
Wavelength (±0,01nm)
Absorbance
590,96
0,04657
593,92
0,04672
596,88
0,04602
599,84
0,04511
602,8
0,04345
605,76
0,04209
608,72
0,04030
611,68
0,03910
614,64
0,03909
Absorbance: 1,10365-0,04345= 1,06020

7)     Culture #7 (0,0333g/ml)
Solution
Bacteria culture
Wavelength (±0,01nm)
Absorbance
590,96
0,06241
593,92
0,06271
596,88
0,06072
599,84
0,05984
602,8
0,05774
605,76
0,05526
608,72
0,05325
611,68
0,05183
614,64
0,05161
Wavelength (±0,01nm)
Absorbance
590,96
1,16439
593,92
1,15925
596,88
1,14791
599,84
1,14328
602,8
1,13782
605,76
1,14632
608,72
1,13356
611,68
1,13724
614,64
1,11817
Absorbance: 1,13782-0,05774= 1,08008